I stained my work station with Coomassie blue solution during my SDS-PAGE protocol. I have been cleaning but the solution seems to can't be removed. What should I use to remove all these stains?
Asked
Active
Viewed 187 times
2
-
What is the surface of the work-station made of? How long have the stains been there? What are you using to clean the stains? – ananta May 31 '23 at 10:19
-
1What is a SDS-PAGE protocol ? – Maurice May 31 '23 at 12:07
-
Coomassie Blue is colorless at pH 1 - 3. So by adding a $\ce{HCl}$ solution diluted to about 0.01 M, it should make the blue dye colorless. – Maurice May 31 '23 at 12:14
-
4The color will come back once HCl evaporates. A very dilute solution of bleach (used as per manufacturer's and lab instructions) should remove dye stains. – AChem May 31 '23 at 12:34
-
Use organic solvents, like ethanol, DMSO, etc. – ananta May 31 '23 at 14:23
-
Why bother at least you will know its yours :) – porphyrin May 31 '23 at 18:00